prefix of negation, Old English un-, from Proto-Germanic *un- (source also of Old Saxon, Old Frisian, Old High German, German un-, Gothic un-, Dutch on-), from PIE *n- (source of Sanskrit a-, an- "not," Greek a-, an-, Old Irish an-, Latin in-), combining form of PIE root *ne- "not." Often euphemistic (such as untruth for "lie").
The most prolific of English prefixes, freely and widely used in Old English, where it forms more than 1,000 compounds. It underwent a mass extinction in early Middle English, but emerged with renewed vigor 16c. to form compounds with native and imported words. It disputes with Latin-derived cognate in- (1) the right to form the negation of certain words (indigestable/undigestable, etc.), and though both might be deployed in cooperation to indicate shades of meaning (unfamous/infamous), typically they are not.
It also makes words from phrases (such as uncalled-for, c. 1600; undreamed-of, 1630s; uncome-at-able, 1690s; unputdownable, 1947, of a book; un-in-one-breath-utterable, Ben Jonson; etc., but the habit is not restricted to un-; such as put-up-able-with, 1812). As a prefix in telegramese to replace not and save the cost of a word, it is attested by 1936.
prefix of reversal, deprivation, or removal (as in unhand, undo, unbutton), Old English on-, un-, from Proto-Germanic *andi- (source also of Old Saxon ant-, Old Norse and-, Dutch ont-, Old High German ant-, German ent-, Gothic and- "against"), from PIE *anti "facing opposite, near, in front of, before, against" (from PIE root *ant- "front, forehead," with derivatives meaning "in front of, before").
More or less confused with un- (1) through similarity in the notions of "negation" and "reversal;" an adjective such as unlocked might represent "not locked" (un- (1)) or the past tense of unlock (un- (2)).